| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184797 |
| Chromosome: | chromosome 5 |
| Location: | 2479182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235550 | OPR125 | OctotricoPeptide Repeat protein 125; (1 of 3) IPR016030 - Adenosylcobalamin biosynthesis, ATP:cob(I)alamin adenosyltransferase-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGCAAGCCTCAGCCCCAGCACCAACT |
| Internal bar code: | GCGCGGATTCGATGTTGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 997 |
| LEAP-Seq percent confirming: | 99.1842 |
| LEAP-Seq n confirming: | 1459 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCGTAGGTCACGGTACT |
| Suggested primer 2: | GTTGCAACATGCGTGTCTCT |