| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184810 |
| Chromosome: | chromosome 12 |
| Location: | 5509664 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531050 | RAA3,OPR67 | psaA mRNA trans-splicing factor; (1 of 45) IPR013584 - RAP domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCAGGACGTGGGAGGCGGCTCAGGCG |
| Internal bar code: | TGACAACCTCGACCATGTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGTCGATGCCTCAACAA |
| Suggested primer 2: | AACCAATTGTAGCACTGCCC |