| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184823 |
| Chromosome: | chromosome 16 |
| Location: | 2710178 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662350 | TSPS1,TPS2 | (1 of 3) 2.4.1.15 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase; Trehalose-6-phosphate synthase, class I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTTGAGCAAGGGGCAGGGGTTGCGGGC |
| Internal bar code: | TCGAAGGCCGCACTCCGATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.6426 |
| LEAP-Seq n confirming: | 9200 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATGATGTGTTCGTCACT |
| Suggested primer 2: | ATCTGTTGCTGCTGCTGTTG |