| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184828 |
| Chromosome: | chromosome 2 |
| Location: | 4342475 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099300 | ANK21 | (1 of 15) PF13857 - Ankyrin repeats (many copies) (Ank_5); Predicted protein with ankyrin repeats | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCAGCTCCTCCGCAGCCAGCGCGTCC |
| Internal bar code: | TTTAAGTTTGTTACATGATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAATAGGGGACTCATCAA |
| Suggested primer 2: | CAGCCTTCCGTCTCAGAATC |