| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184832 |
| Chromosome: | chromosome 12 |
| Location: | 4667470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523150 | PFD5 | Prefoldin alpha subunit; (1 of 1) K04797 - prefoldin alpha subunit (pfdA, PFDN5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGCTGCCGAGTCACGGTGGTTCTCACG |
| Internal bar code: | TTGATCGACGTCGTCTGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 99.0915 |
| LEAP-Seq n confirming: | 9053 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCAGCGGTTAATGGAGA |
| Suggested primer 2: | TCTGGGATTCTGGGAATCAG |