Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.184843 |
Chromosome: | chromosome_8 |
Location: | 1916676 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g367250 | FAP176 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACCTACCGAGTTGCCATGTTTGTAGTGTT |
Internal bar code: | TGGGTAGGAGGGCAGAAGGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 492 |
LEAP-Seq percent confirming: | 99.435 |
LEAP-Seq n confirming: | 528 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGCAGGTGAGAGGTGAGTG |
Suggested primer 2: | GAACAGCAGTTGTCCCCATT |