| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184853 |
| Chromosome: | chromosome 1 |
| Location: | 6680407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTACCTGTCGCCGCACACGGTGACGCC |
| Internal bar code: | GTCGAGGCATTGCTCCGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 63.0435 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGTGTCTTCCCTCAAC |
| Suggested primer 2: | AAAGGAGAAAGGCACAGCAA |