| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184876 |
| Chromosome: | chromosome 17 |
| Location: | 5108478 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735021 | (1 of 6) PTHR10582 - TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGCTCTCGCCATCCCCCACACACAAGT |
| Internal bar code: | GATGCGGCGCTCGGGAGTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 97.482 |
| LEAP-Seq n confirming: | 12892 |
| LEAP-Seq n nonconfirming: | 333 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTACCTGCCCAGTCCTTG |
| Suggested primer 2: | GAATGGATTCGGCTTTGAGA |