| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184919 |
| Chromosome: | chromosome 7 |
| Location: | 2531873 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGTCGTCCTGCCCCTGACGATCCGGA |
| Internal bar code: | TGGTCAAGTTGGGGTCGAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 99.3019 |
| LEAP-Seq n confirming: | 569 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCTACTCCTCTTCAGTG |
| Suggested primer 2: | ATGAGAGAAGAGCGAGCGAG |