| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184938 |
| Chromosome: | chromosome 6 |
| Location: | 2429268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268501 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGGCAGGATCGTGAGCCCCCTACCGCA |
| Internal bar code: | GGCACTGGCCGCACAACCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 99.536 |
| LEAP-Seq n confirming: | 429 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTCGACGTGACTTTG |
| Suggested primer 2: | ACCAGCCATATCAGGTCGAG |