Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.184975 |
Chromosome: | chromosome 16 |
Location: | 1371781 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g651950 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCACCAGTGTATCTATTGAGCGCGCCT |
Internal bar code: | TCCGATGCGCTGGGTGATGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 66 |
LEAP-Seq percent confirming: | 99.4624 |
LEAP-Seq n confirming: | 185 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTCAGCCTTACGTAGCACC |
Suggested primer 2: | GAACAATGTCGGTTCGGAGT |