| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184990 |
| Chromosome: | chromosome 17 |
| Location: | 6240188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742250 | NCG9,CDKG2 | Cyclin dependent kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATAACGAGTGATGCTTGCGCTGCTGTCT |
| Internal bar code: | CTTCGCTTCCTGTGTGTCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 99.2331 |
| LEAP-Seq n confirming: | 1941 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTGTATCCCAACCCCTG |
| Suggested primer 2: | ACAGGTGGTGACGCTCTTCT |