| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185068 |
| Chromosome: | chromosome 9 |
| Location: | 734242 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402602 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCCCCAGCGCCTGCGCCTGAGACTTCA |
| Internal bar code: | GGTAGATGCAGTGCACGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 88.909 |
| LEAP-Seq n confirming: | 1475 |
| LEAP-Seq n nonconfirming: | 184 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTGTATGACACCGTATG |
| Suggested primer 2: | ATTACGACAAAGCCCGACAC |