| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185073 |
| Chromosome: | chromosome 1 |
| Location: | 2991743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCGCCCCGCACCTCACTCCTACTCTCC |
| Internal bar code: | AACGCCAACCGGCTGTGGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 99.816 |
| LEAP-Seq n confirming: | 2170 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAGGAGGACATCATCA |
| Suggested primer 2: | TTGCGTACATGGCATTTTGT |