| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185086 |
| Chromosome: | chromosome 11 |
| Location: | 351901 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467574 | (1 of 1) PTHR12170//PTHR12170:SF3 - MACROPHAGE ERYTHROBLAST ATTACHER-RELATED // REQUIRED FOR MEIOTIC NUCLEAR DIVISION 5 HOMOLOG B (S. CEREVISIAE) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCTTGGCAGGCAATCATGATCTTCTC |
| Internal bar code: | GCCCGGCTCAATAAGCAAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 97.6384 |
| LEAP-Seq n confirming: | 1199 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCCCCTACTGCCCTAT |
| Suggested primer 2: | CCGTCAACAACAACATCCTG |