Insertion junction: LMJ.RY0402.185088_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.185088
Chromosome:chromosome 7
Location:1505995
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g324000 NUP160 Nucleoporin 160; (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTAGGTAGGACGCTTGGTGGGGAGGAGGG
Internal bar code:CGTCTACCGTTGGCGGATCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:387
LEAP-Seq percent confirming:99.5828
LEAP-Seq n confirming:10265
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCAGCTTGACCTTCTTC
Suggested primer 2:TCTGGGTCTCGGGTAATGTC