| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185088 |
| Chromosome: | chromosome 7 |
| Location: | 1506019 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324000 | NUP160 | Nucleoporin 160; (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACCTAGCTAGCCACCTCGCCACGCCC |
| Internal bar code: | TTAAGGAATCGGGTGCTGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGGTCTCGGGTAATGTC |
| Suggested primer 2: | GCACCAGCTTGACCTTCTTC |