Insertion junction: LMJ.RY0402.185101_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.185101
Chromosome:chromosome 7
Location:3015568
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g333400 MMP23 Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTCGAAGAGAGCATCCTGTCATCCCCA
Internal bar code:GGTAGCGTCTACCTGTAGGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:231
LEAP-Seq percent confirming:99.8255
LEAP-Seq n confirming:572
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGAATGGGGCAGAAACACAC
Suggested primer 2:AGTGGTCCACAACATGCAAA