Insertion junction: LMJ.RY0402.185110_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185110
Chromosome:chromosome 3
Location:7341963
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g206550 CPLD2,PGP5 (1 of 2) PTHR18901//PTHR18901:SF9 - 2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2 // SUBFAMILY NOT NAMED; conserved expressed protein with hydrolase motif intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGATGATAGCTCCACATTATATCCGTGA
Internal bar code:GGTTGGTCGTGCACAGTTCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:99.9203
LEAP-Seq n confirming:2508
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TATCTTGTAATGCTCGCCCC
Suggested primer 2:GCTCGACGAAACTCTTTTGG