| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185137 |
| Chromosome: | chromosome 9 |
| Location: | 223663 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386753 | (1 of 1) PF04689 - DNA binding protein S1FA (S1FA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACTTATGCGGTGACTGAGGCATGTATA |
| Internal bar code: | GGTGGACAGGAGTTCTCCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 99.7167 |
| LEAP-Seq n confirming: | 1408 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAGAAGGCAGCATCGTG |
| Suggested primer 2: | CTATTTCGTTCCAGCGCTTC |