| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185139 |
| Chromosome: | chromosome 1 |
| Location: | 6816195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049132 | (1 of 1) PTHR23097//PTHR23097:SF105 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGACGCGGGCAGGTGCGGGATTACGC |
| Internal bar code: | GCCGAGTTCGTCACCGGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1093 |
| LEAP-Seq percent confirming: | 99.7926 |
| LEAP-Seq n confirming: | 2406 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGTCTTCGTCCGAGTC |
| Suggested primer 2: | TCACGCTCTGATGCAAAAAC |