| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185139 |
| Chromosome: | chromosome 7 |
| Location: | 1767896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325720 | (1 of 5) IPR000104//IPR001054//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGAGGCGAAGCCCCCCAAGAACACCCT |
| Internal bar code: | AGTTCTGCTTTCGTAATACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 94.9184 |
| LEAP-Seq n confirming: | 3026 |
| LEAP-Seq n nonconfirming: | 162 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTTGCAGTAATCTCACA |
| Suggested primer 2: | TGTTGCCAAAGATCAAGTCG |