Insertion junction: LMJ.RY0402.185144_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185144
Chromosome:scaffold 36
Location:2209
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre36.g759597 (1 of 2) IPR001841//IPR013083//IPR024766 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, RING-H2-type 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCTGGCATCGATGATAGCAGCGGCAAC
Internal bar code:GCGCGTAAACGGTCGCACATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:68
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGGATGTGTTCTGTCAC
Suggested primer 2:AAATGTTGCAAGGCGAAGTT