| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185162 |
| Chromosome: | chromosome 7 |
| Location: | 6103607 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355500 | (1 of 2) PF03226 - Yippee zinc-binding/DNA-binding /Mis18, centromere assembly (Yippee-Mis18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATGAGATAAGAGATGAGGGGTTGTAGA |
| Internal bar code: | TTTAGACCAGTGGGGCTCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 98.9754 |
| LEAP-Seq n confirming: | 5023 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCAAGCCAAGCTTACTG |
| Suggested primer 2: | GAGGGCAAGGTCATACTGGA |