| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185187 |
| Chromosome: | chromosome 3 |
| Location: | 4591863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176961 | (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCGCCCGCCAACACCACGTGCCTGGAC |
| Internal bar code: | GGCCCACTTCGACCTTACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 99.7248 |
| LEAP-Seq n confirming: | 1087 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAAGATGGTGGGTCCGT |
| Suggested primer 2: | TGAGAAGCAGAGGGACCAGT |