Insertion junction: LMJ.RY0402.185190_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.185190
Chromosome:chromosome 13
Location:4538110
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g602750 (1 of 5) PTHR32467:SF15 - AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMZ-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGGGTCTCGGGGAACTTAACTCCCACG
Internal bar code:TCACGTGGCCACCAGGGTAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:27
LEAP-Seq percent confirming:99.8008
LEAP-Seq n confirming:501
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTTTCACCACGGTCCTC
Suggested primer 2:TTCCTCCCCCTCTCCTTAAA