Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.185208 |
Chromosome: | chromosome 2 |
Location: | 3239739 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g095076 | ROC39,MFT10 | Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCACCGTGCACCGTGCACCGTGCGTTG |
Internal bar code: | GGATGGATTGGTGTCTAAGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 438 |
LEAP-Seq percent confirming: | 99.5375 |
LEAP-Seq n confirming: | 3659 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGAGCGTTTCGTTCTTAAC |
Suggested primer 2: | ATGGGCACTCCTTCGTACAC |