| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185216 |
| Chromosome: | chromosome 8 |
| Location: | 1861583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACGCTTTCCTACTTGACCCTGCCATTG |
| Internal bar code: | ATCTCGGGAAGTCGCCACGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 98.8339 |
| LEAP-Seq n confirming: | 2458 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACGACCCGTATTCAGAC |
| Suggested primer 2: | TTTGCCAGCTTTCATCAGTG |