Insertion junction: LMJ.RY0402.185228_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185228
Chromosome:chromosome 1
Location:3737050
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g024050 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACAAAGTGTTCAACATAGCGGTCGACTC
Internal bar code:ATGACCTCGGGAGAGTGGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1165
LEAP-Seq percent confirming:99.0931
LEAP-Seq n confirming:4480
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGAAGGATGGAGGATCAG
Suggested primer 2:AGAATTGGGACGTGAAGGTG