| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185264 |
| Chromosome: | chromosome 5 |
| Location: | 2316040 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234801 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATAACGCTTAAGCCAAAGTGCGCATGAC |
| Internal bar code: | GTTGCGCAACGGACTGGAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.7157 |
| LEAP-Seq n confirming: | 1754 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGACCGACTCCAACCTC |
| Suggested primer 2: | GTCCTGCGGACAAGAGTAGC |