| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185271 |
| Chromosome: | chromosome_3 |
| Location: | 7552547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g205050 | DGTT4,CGLD24,DOT1 | Diacylglycerol O-acyltransferase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCGAACAAGTAGCCGCGGTCAGTGCGGA |
| Internal bar code: | GGCTCACTGACCTGGTAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 99.3613 |
| LEAP-Seq n confirming: | 11668 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGCGACTGTTCTGTGAAA |
| Suggested primer 2: | ACCAAACAACGCCTAACGAC |