| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185271 |
| Chromosome: | scaffold 19 |
| Location: | 62299 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750497 | RAP-domain containing protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCGTGTGTTTGGCGCGGTGCCCAGTA |
| Internal bar code: | TGTGTATAGACGCGGAAAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 94.4954 |
| LEAP-Seq n confirming: | 206 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCAGGATGTGCTATTTG |
| Suggested primer 2: | TTGGTTTATAGCCAGGTCGG |