Insertion junction: LMJ.RY0402.185308_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185308
Chromosome:chromosome 7
Location:2571845
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g329882 VGK1,VGKC Ion transport protein; (1 of 1) PTHR10217//PTHR10217:SF503 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATCTTGTCACCGCTCAGGAAATTGTTAA
Internal bar code:GGCACTACCGCGGCGCGAGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:485
LEAP-Seq percent confirming:99.8102
LEAP-Seq n confirming:526
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TAATCCTCGACCCAGATTGC
Suggested primer 2:ACTCTGTCCAGGGACACCAC