| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185323 |
| Chromosome: | chromosome 13 |
| Location: | 4745492 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604905 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCGGACTCGGAGGCCTTGTAGCTGCA |
| Internal bar code: | GTTTGCGTTGCAATCTCCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 95.1276 |
| LEAP-Seq n confirming: | 1230 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAAACCAACTTGCCTGG |
| Suggested primer 2: | GCAAACAACCGCCAGTATTT |