Insertion junction: LMJ.RY0402.185342_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185342
Chromosome:chromosome 8
Location:4586867
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g383101 FAP324 (1 of 1) PF00041//PF13418 - Fibronectin type III domain (fn3) // Galactose oxidase, central domain (Kelch_4); Flagellar Associated Protein 324 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGGCCAGTGACGCGGAAGCGGCTCTCG
Internal bar code:TCGGTGCATGACGTTGCACTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:63
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGTATGCTTTGCTGCTG
Suggested primer 2:ACGTTGGGATTGGAGTGAAG