| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185401 |
| Chromosome: | chromosome 16 |
| Location: | 7213199 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681914 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGCGAGCAGCAGCAGAGGCGGTTGCGA |
| Internal bar code: | TTCGAGCGGTGGAGTGCGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 85.6749 |
| LEAP-Seq n confirming: | 622 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCAGCTACTGAGCACAC |
| Suggested primer 2: | GCGTCATACGCTTAGCACAA |