Insertion junction: LMJ.RY0402.185406_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.185406
Chromosome:chromosome 10
Location:6247255
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g464800 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGTCGCTGACGCAGCCCGTCCACCGCC
Internal bar code:AAATGTCAACACGGGGCAGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:934
LEAP-Seq percent confirming:99.4407
LEAP-Seq n confirming:4267
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTACTAACGTCCACGGC
Suggested primer 2:GCTGATGTTTGAGCTGGACA