| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185409 |
| Chromosome: | chromosome 5 |
| Location: | 898981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247450 | CGL56,RDP5 | Putative rhodanese-like protein; (1 of 1) PTHR34209//PTHR34209:SF1 - FAMILY NOT NAMED // RHODANESE/CELL CYCLE CONTROL PHOSPHATASE SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGACCTACCGTAGGATAGGATTGCGGG |
| Internal bar code: | GGGAGGTTCGGCCATCTTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.7596 |
| LEAP-Seq n confirming: | 2905 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGCTTTTCCAAACATCC |
| Suggested primer 2: | GCAGGGGTGGTCCTACTACA |