| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185420 |
| Chromosome: | chromosome 2 |
| Location: | 1250951 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082550 | ZEP,ZEP1 | Zeaxanthin epoxidase 1; (1 of 1) K09838 - zeaxanthin epoxidase (ZEP, ABA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAAACCAAAATTAAATCGCATATGAT |
| Internal bar code: | GACTCGAACGTAAAAAGTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 99.6756 |
| LEAP-Seq n confirming: | 1229 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCTCTCTTGTGAGGAGT |
| Suggested primer 2: | TGGGGCATACAACAGTACGA |