| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185422 |
| Chromosome: | chromosome 3 |
| Location: | 7434454 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205850 | MAE6,MFT6 | MATE efflux family protein; (1 of 3) PTHR11206//PTHR11206:SF95 - MULTIDRUG RESISTANCE PROTEIN // MULTI ANTIMICROBIAL EXTRUSION FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACATCGGTTTCCCGACTGTCCCCTGGGC |
| Internal bar code: | CGGCGACGGGCTAGTGAATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 99.7803 |
| LEAP-Seq n confirming: | 2271 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCAGCTCACATATTGGAT |
| Suggested primer 2: | GCAACCTCTGGCAAGACTTC |