| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185425 |
| Chromosome: | chromosome 14 |
| Location: | 3058834 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628533 | CYG3 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGCTGTCAGGTTGATGAGCTGAAGTG |
| Internal bar code: | CTCATGGTGATACACGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.6849 |
| LEAP-Seq n confirming: | 3164 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCGGACTTGGATCAGGA |
| Suggested primer 2: | CACGTAGCATAGCCTGCAAA |