| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185431 |
| Chromosome: | chromosome_8 |
| Location: | 4611159 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g383400 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTTGCTTTGTTGCTTGCCGTTCATCAGG |
| Internal bar code: | GCCACATGGCCTAACCGAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 97.8233 |
| LEAP-Seq n confirming: | 764 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACAATCCAATCTCACG |
| Suggested primer 2: | AATTCCCGATAAAGCGGTCT |