| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185442 |
| Chromosome: | chromosome_3 |
| Location: | 7932716 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g202200 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACAACGCGCAGCTGTCCAACCAGGTAGA |
| Internal bar code: | ATTGGCATTGCGTGGGGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.7819 |
| LEAP-Seq n confirming: | 915 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGGTGCTGGTGAAGAGA |
| Suggested primer 2: | ACCGGTATGTTACGGTGAGC |