Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.185442 |
Chromosome: | chromosome_4 |
Location: | 2929145 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g224650 | FAP296 | Flagellar Associated importin alpha-like protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCACAACCGACCTGGGCAGCTCGCCGCAG |
Internal bar code: | GATGGGGAATGTGAGGTGTCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 946 |
LEAP-Seq percent confirming: | 99.7694 |
LEAP-Seq n confirming: | 4759 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTCTGTGTCAGCCATCGTC |
Suggested primer 2: | TCGATTGCTGCTTTATGTCG |