| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185454 |
| Chromosome: | chromosome 1 |
| Location: | 554097 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003150 | protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTTACCCACCATCGTGCGGCGTTGCGG |
| Internal bar code: | GTGCGTGGTTAGCACCGATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 78.9272 |
| LEAP-Seq n confirming: | 1030 |
| LEAP-Seq n nonconfirming: | 275 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTCTAGCCTACGCAAC |
| Suggested primer 2: | AGACAGGTTTCGTTTGGACG |