| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185456 |
| Chromosome: | chromosome 6 |
| Location: | 1679799 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261150 | BIO2,BIOB | (1 of 1) K01012 - biotin synthase (bioB); Biotin synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATTGTCAAGGTGGAGGGAAGAAGACAA |
| Internal bar code: | CAGCTCCCCTCCAAGGCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGGTGTGTGTGTGTGTA |
| Suggested primer 2: | TTGCGTGTTGATTTTGGTGT |