| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.185475 |
| Chromosome: | chromosome 6 |
| Location: | 8342352 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306400 | (1 of 1) 4.4.1.14 - 1-aminocyclopropane-1-carboxylate synthase / S-adenosyl-L-methionine methylthioadenosine-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTGCGGCCTCGGCTGCGGCGTTGAGC |
| Internal bar code: | AACGGGCAACGGGAGCGGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 98.4095 |
| LEAP-Seq n confirming: | 3960 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGTAGATCTCGTCACTG |
| Suggested primer 2: | GTGGGTGTGGATTTGGGTAG |