| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.185486 |
| Chromosome: | chromosome 9 |
| Location: | 7697591 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415650 | FAP351 | Flagellar Associated Protein 351; (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAATGGCACCCCCCCCCCGCACGACGGA |
| Internal bar code: | TAGTCGTCGCCCTTTGCATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 99.3492 |
| LEAP-Seq n confirming: | 1374 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGGACTGCTCCTTCCAC |
| Suggested primer 2: | TAGCCGTTGCACTGTTTGTC |