| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185512 |
| Chromosome: | chromosome 2 |
| Location: | 4065324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097300 | (1 of 1) IPR030184 - WAT1-related protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGTACATACCCGTGCTTGCGCCCACCA |
| Internal bar code: | GCTAGTTAGGTTATGTTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 97.9554 |
| LEAP-Seq n confirming: | 527 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATACGTCCTGTCCCAACT |
| Suggested primer 2: | AAATACCGTCGTTGACCAGC |