| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.185528 |
| Chromosome: | chromosome 12 |
| Location: | 232078 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485200 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGCTTCGACACCAGCCATCGCTCCGAG |
| Internal bar code: | TCAGTACCCAGGGCGGAGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 98.7682 |
| LEAP-Seq n confirming: | 2245 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGTTCTGAGGAGATTGC |
| Suggested primer 2: | ACCACGCAGTAGGGATCTTG |